Polimorfismo da glutationiona-S-transferase P1 e marcadores de estresse oxidativo em mulheres infectadas por papilomavírus humano. | Glutathione-S-transferase P1 polymorphism and oxidative stress markers in women infected by human papilomavirus.
Resumo
Objetivo: analisar o polimorfismo genético de GSTP1 localizado no cromossomo 11.q13.2, exon 5 (Ile105Val) e associar a marcadores de estresse oxidativo e infecção por HPV. Métodos: o DNA foi extraído de raspados cervicais para identificação do HPV por PCR e genotipagem utilizando HPV-Screening PapilloCheck® e de sangue total para análise do polimorfismo por RFLP-PCR. O estresse oxidativo foi analisado através dos níveis séricos das espécies reativas ao ácido tiobarbitúrico, grupos carbonil, grupos tiol e atividade da catalase. Resultados: 75% das mulheres HPV positivas apresentaram infecções simples e os tipos virais 16 e 45 foram mais frequentes. 56,25% das mulheres apresentaram o genótipo variante Ile105Val GSTP1 (Ile/Val ou Val/Val) com prevalência de heterozigotos (43,75%). Entre as mulheres HPV positivas, a presença do variante alélico levou a um maior risco de desenvolver lesões cervicais (OR = 29,44). Mulheres infectadas com HPV (especialmente de alto risco) apresentaram menor atividade da catalase e aumento dos grupos carbonila. Considerando a presença dos genótipos variantes Ile/Val e Val/Val, as mulheres infectadas apresentaram aumento dos marcadores de estresse oxidativo e as mulheres HPV negativas apresentaram diminuição da capacidade antioxidante. Conclusão: estes achados sugerem uma possível influência do genótipo variante GSTP1 no aumento do estresse oxidativo e suscetibilidade a lesões cervicais em mulheres infectadas com HPV.
Palavras-chave: gstp1; espécies reativas de oxigênio; hpv; lesão cervical.
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Abstract
Objective: to analyze the genetic polymorphism of GSTP1 located on chromosome 11q13.2 exon 5 (Ile105Val) and its association with oxidative stress markers and HPV infection. Methods: DNA were extracted from cervical scrapes for HPV identification by PCR, genotyping using HPV-Screening PapilloCheck®, and from whole blood for analysis of polymorphisms by RFLP-PCR. Oxidative stress was analyzed by measuring the serum levels of thiobarbituric acid reactive species, carbonyl groups, thiol groups, and catalase activity. Results: of all HPV-positive women, 75% had simple infections. Viral types 16 and 45 were the most frequent. A total of 56.25% of women had variant Ile105Val GSTP1 genotype (Ile/Val or Val/Val) with a heterozygous prevalence (43.75%). Among the HPV-positive women, the presence of the allelic variant led to a higher risk of developing cervical lesions (OR = 29.44). Women infected with HPV (especially the high-risk type) showed lower catalase activity and an increase in carbonyl groups. Considering the presence of variant genotypes Ile/Val and Val/Val, the infected women presented an increase in some oxidative stress markers, and HPV-negative women showed a decreased antioxidant capacity. Conclusion: these results indicate the potential influence of variant GSTP1 genotype on increased oxidative stress and susceptibility to cervical lesions in women infected with HPV.
Keywords: gstp1; reactive oxygen species; HPV; cervical injury.
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Artigo em InglêsReferências
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