REPERCUSSÕES EM CURTO PRAZO DA LESÃO COMPRESSIVA DO NERVO ISQUIÁTICO DE RATOS, TRATADOS COM NATAÇÃO, SOBRE A CARTILAGEM ARTICULAR DA TÍBIA DE RATOS WISTAR

Izabela Rodrigues Camilo, Gladson Ricardo Flor Bertolini, Jean Carlos Debastiani, Lucineia de Fátima Chasko Ribeiro, Rose Meire Costa

Resumo


RESUMO

Objetivo: analisar, em curto período, os possíveis efeitos de uma lesão nervosa isquiática nos parâmetros histomorfométricos da cartilagem articular da tíbia de ratos Wistar, bem como se a natação com sobrecarga gera algum tipo de repercussão. Métodos: 24 ratos foram divididos em 3 grupos (n=8/grupo), sendo: grupo controle (GC), grupo lesão (GL) e grupo lesão+natação (GLN). Três dias após a cirurgia de compressão nervosa, o grupo GLN foi submetido ao exercício de natação com carga, durante 21 dias. Após a eutanásia, as articulações do joelho foram coletadas, fixadas e seguiram o protocolo para emblocamento em parafina. As lâminas coradas com hematoxilina-eosina foram analisadas quanto aos parâmetros morfológicos de espessura da cartilagem articular e da epífise de crescimento, além de contagem do número de condrócitos. Resultados: para nenhuma das variáveis analisadas, houve diferença significativa entre os três grupos. Conclusão: os modelos de lesão nervosa e exercícios não foram capazes de produzir alterações significativas na cartilagem das tíbias dos animais.

Palavras-chave: Compressão nervosa; cartilagem articular; exercício terapêutico; natação.

 

Abstract: Objective: to analyze, in a short period of time, the possible effects of an ischial nerve lesion on the histomorphometric parameters of the tibial joint cartilage of Wistar rats, as well as whether overloaded swimming generates some kind of repercussion. Methods: 24 rats were divided into 3 groups (n=8/group), being: control group (CG), lesion group (LG) and lesion+swimming group (LSG). Three days after nerve compression surgery, the LSG underwent loaded swimming exercise for 21 days. After euthanasia, the knee joints were collected, fixed and followed the protocol for paraffin embedding. The slides stained with hematoxylin-eosin were analyzed for the morphological parameters of joint cartilage and growth epiphysis thickness, in addition to counting the number of chondrocytes. Results: for none of the variables analyzed were there significant differences between the three groups. Conclusion: The models of nerve damage and exercise were not able to produce significant changes in the tibia cartilage of the animals.

Key words: Nerve crush; articular cartilage; exercise therapy; swimming.

 

Figshare DOI: 10.6084/m9.figshare.11874045


Palavras-chave


Compressão nervosa; cartilagem articular; exercício terapêutico; natação.

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Referências


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Revista Brasileira de Pesquisa em Ciências da Saúde - RBPeCS - ISSN: 2446-5577


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